BOTANY Biotechnology Mock Test

🟥 BOTANY Biotechnology Mock Test

🟥 QUESTIONS : 20

🟥 TIME : 20 MINS

1 / 20

1)

The correct order of steps in Polymerase Chain Reaction (PCR) is : ]
-
-

A) Extension, Denaturation, Annealing

B) Annealing, Extension, Denaturation

C) - Denaturation, Annealing, Extension

D) - Denaturation, Extension, Annealing

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2) A selectable marker is used to

A) Mark a gene on a chromosome for isolation using restriction enzyme.

B) Help in eliminating the non-transformants, so that the transformants can be regenerated.

C) Identify the gene for a desired trait in an alien organism.

D) - Select a suitable vector for transformation in a specific crop.

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3) Which of the following statements does not hold true for restriction enzyme ? ]

A) - It recognizes a palindromic nucleotide sequence.

B) - It is an endonuclease.

C) - It is isolated from viruses.

D) - It produces the same kind of sticky ends in different DNA molecules.

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4) : Who among the following was awarded the Nobel Prize for the development of PCR technique ? ]

-

A) - Herbert Boyer

B) - Kary Mullis

C) Arthur Kornberg

D) - Hargovind Khurana

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5) Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts ? ]

A) - Laboratory flask of largest capacity

B) - A stirred-tank bioreactor without inlets and outlets

C) - A continuous culture system

D) - Any of the above

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6) A bacterial cell was transformed with a recombinant DNA that was generated using a human gene; however, the transformed cells did not produce the desired protein. The reasons could be : ]

-

A) - Human gene may have intron which the bacteria cannot process.

B) - Human protein is formed but degraded by bacteria.

C) Amino acid codons for humans and bacteria are different.

D) - All of the above

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7) Which of the following steps are catalysed by Taq polymerase in a PCR reaction ? ]

A) - Denaturation of template DNA

B) - Annealing of primers to template DNA

C) - Extension of primer end on the template DNA

D) - All of the above

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8) Which of the following is not a source of restriction endonuclease ?

A) - Escherichia coli

B) Haemophilus influenzae

C) Agrobacterium tumefaciens

D) - Bacillius amyloliquifaciens

9 / 20

The role of DNA ligase in the construction of a recombinant DNA molecule is : ]
-

9) -.

A) Formation of phosphodiester bond between two DNA fragments.

B) - Formation of hydrogen bonds between sticky ends of DNA fragments.

C) Ligation of all purine and pyrimidine bases

10 / 20

10)

: Significance of ‘heat shock’ method in bacterial transformation is to facilitate the : ]
-
.

A) Binding of DNA to the cell wall.

B) - Uptake of DNA through transient pores in the bacterial cell wall. -

C) Expression of antibiotic resistance gene.

D) - Uptake of DNA through membrane transport proteins

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11) An antibiotic resistance gene in a vector usually helps in the selection of ?

A) Competent cells

B) Transformed cells -

C) Recombinant cells

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12) : Which of the following has popularised the PCR (Polymerase Chain Reactions) ? ]

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A) - Easy availability of DNA template

B) - Availability of ‘Thermostable’ DNA polymerase

C) Availability of synthetic primers -

D) Availability of cheap deoxyribonucleotides

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13) : While isolating DNA from bacteria, which of the following enzymes is not used ? ]

A) - Lysozyme

B) - Ribonuclease

C) - Deoxyribonuclease

D) - Protease

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14) The most important feature in a plasmid to be used as a vector is : ]

A) - Presence of a selectable marker

B) - Origin of replication (ori)

C) - Its size

D) - Presence of sites for restriction endonuclease

15 / 20

15) In agarose gel electrophoresis, DNA molecules are separated on the basis of their :

A) Charge only

B) - Both charge and size

C) Charge to size ratio

D) Size only

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16) Which of the following is not required in the preparation of recombinant DNA molecule ?

A) - Restriction endonuclease

B) - DNA ligase

C) - E. coli

D) DNA fragments

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17) 'Restriction’ in Restriction enzyme refers to

A) - Cleaving of phosphodiester bond in DNA by the enzyme.

B) - Cutting of DNA at specific position only.

C) - Prevention of the multiplication of bacteriophage in bacteria.

D) - All of the above

18 / 20

18) Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis ?

A) DNA can be seen in visible light

B) DNA can be seen without staining in visible light.

C) - Ethidium bromide stained DNA can be seen in visible light.

D) Ethidium bromide stained DNA can be seen under exposure to UV light.

19 / 20

: The transfer of genetic material from one bacterium to another through the mediation of a vector like virus is termed as : ]
-

19) -

A) Transduction

B) - Conjugation

C) Transformation

D) - Translation

20 / 20

20) An enzyme catalysing the removal of nucleotides from the ends of DNA is

-

A) Endonuclease

B) - Exonuclease

C) - DNA ligase

D) Hind-II

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BOTANY Biotechnology Mock Test

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