BOTANY Biophysics Mock Test 2

BOTANY Biophysics Mock Test

🟥 Biophysics Mock Test

🟥 QUESTIONS :35

🟥 TIME : 20 MINS

1 / 35

1) If proteins are separated according to their electrophoretic mobility then the type of electrophoresis is:

A) Free flow electrophoresis

B) Affinity Electrophoresis

C) SDS PAGE

D) Electro focusing

2 / 35

2) ELISA can be used as diagnostic tool in

A) medicine

B) plant pathology

C) quality control

D) all of these

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3)

Which of the following is used in uranium enrichment?
a)
b)
c)
d)

A) Tubular centrifuge

B) Disk-stack centrifuge

C) Gas centrifuge

D) Zippe-type centrifuge

4 / 35

4) Image formation in electron microscope is based on ___________________________

A) column length

B) electron number

C) differential scattering

D) specimen size

5 / 35

5) In TEM, the tissue is stained by floating on drops of ______________________

A) hydrocarbons

B) slow-molecular weight stains

C) heavy metal soutions

D) oil immersion

6 / 35

6) Shadow casting is a technique of visualizing ___________________

A) isolated particles

B) mounts

C) shoot tips

D) root tips

7 / 35

7) During TEM, a vacuum is created inside the _________________________

A) specimen

B) column

C) ocular system

D) room of operation

8 / 35

8) The cathode of transmission electron microscope consists of a ____________________

A) tungsten wire

B) bulb

C) iron filament

D) gold wire

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9) . TEM and SEM are the same microscopy techniques.

A) a) True

B) b) False

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10) State true or false:

The charged particles travel faster than light when the Cherenkov radiation passes through water.

A) false

B) true

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11) he reduction in counting efficiency of the scintillation detector is called as __________

A) Disintegration

B) Decay

C) Quenching

D) Reduction

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12)

In liquid scintillation counter, which of the following is a fluorescent substance?
a)
c
d

A) Solvent

B) Solute

C) Crystal

D) Reagent

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13) A quantity of heat required to change the unit mass of a solid substance, from solid state to liquid state, while the temperature remains constant, is known as

A) Latent heat

B) Sublimation

C) Hoafrost

D) Latent heat of fusion

14 / 35

14) Which is not an application of tlc

A) .TLC can be successful used for isolation of vitamin like A,D,andE using silica gel.

B) Identification of drug

C) Neomycin sulphate can be isolating on activated compound.

D) To determination of moisture.

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15) The stationary phase in thin layer chromatography is: –

A.

C.

D

A) Liquid held between glass

B) Glass plate

C) Silica gel

D) . None of the above

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16) The stationary phase in thin layer chromatography is: –

A.

C.

D

A) Liquid held between glass

B) Glass plate

C) Silica gel

D) . None of the above

17 / 35

17) Which is not an application of TLC?

A

B. .

C.

D.

A) .TLC can be successful used for isolation of vitamin like A,D,andE using silica gel.

B) Identification of drug

C) Neomycin sulphate can be isolating on activated compound.

D) To determination of moisture.

18 / 35

18) The principle on which thin layer chromatography is based is that the ____________

A) Different compounds are absorbed on an absorbent to different degrees

B) Different compounds are absorbed on an absorbent to same degrees

C) Different compounds are adsorbed on an adsorbent to different degrees

D) Different compounds are absorbed on an absorbent to same degrees

19 / 35

19) Amino acids detected by spraying the plate with ninhydrin solution is an example of

a)

b)

c)

A) Column chromatography

B) Thin layer chromatography

C) Liquid chromatography

D) Paper chromatography

20 / 35

20) Which of the following electromagnetic radiation is used in ESR?

A) IR radiation

B) Radio waves

C) Microwaves

D) X-radiation

21 / 35

21) Which of the following will NOT show electron spin resonance (ESR)?

(A)

(B)

(C)

(D

A) Free radicals

B) Paramagnetic materials

C) Transition metals

D) Diamagnetic materials

22 / 35

22)

What are the different frequencies obtained as NMR peaks called?
a)
b)
c)
d)

A) Chemical peaks

B) Biological shifts

C) Physical shifts

D) Chemical shifts

23 / 35

23) . NMR spectroscopy cannot be used to study which of the following?

A) Interaction of protein with other molecules

B) Dynamic motion within proteins

C) Protein folding

D) Protein sequence

24 / 35

24) Which of the following is a technique for the determination of the three-dimensional structure of a protein?

A) Gas chromatography

B) Mass spectroscopy

C) Radiotherapy

D) NMR spectroscopy

25 / 35

25) To prepare thin sections the sample must be rigid this is done by

A) freezing the sample

B) drying the sample

C) replacing the aqueous material with an organic monomer

D) covering the sample with a gel

26 / 35

26) The word staining during electron microscopy refers to

A) staining with a dye

B) deposition of metals by chemical reaction

C) formation of complex between metals and certain components of the sample

D) both b and c

27 / 35

27) During electrophoresis the mobility of molecules

A) increases with charge on particles

B) decreases with increase in frictional coefficients

C) both of these

28 / 35

28) Will two molecules having molecular weight and charge have the same mobility

A) yes, because they have same frictional coefficient

B) no, because they have different shapes hence different frictional coefficient

C) both a and b

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29) During replica formation the water in the sample must be removed .this is usually done by

A) using gel

B) freeze-etching

C) critical point method

D) all of these

30 / 35

30) Shadow casting is not successful for smaller molecular because

A) small size of shadow

B) granularity of support film

C) pressure id high

D) both a and b

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31) Mobility of macromolecules during SDS-PAGE

A) icrease with increasing molecular weight

B) decrease with increasing molecular weight

C) no effect of molecular weight

D) increase with increasing change

32 / 35

32) The driving force on each protein molecule during SDS-polyarylamide gel electrophoresis is proportional to

A) number of aminoacid per molecule

B) number of peptide bonds per molecule

C) change per molecule

33 / 35

33) During electrophoresis

A) velocity of molecule is proportional to applied voltage

B) velocity is proportional to the current

C) velocity is infactpropotional to voltage seen by the molecules and not to the applied voltage and the value of voltage across the gel is determined by the current

34 / 35

34) the transfer of DNA from the gels to nitrocellulose membrane is known as

A) Northern transfer

B) southern transfer

C) westrn transfer

D) eastern transfer

35 / 35

35) In partition chromatography the broadening of peaks can be reduced by

A) very small genes

B) rapid seperation

C) slow seperation and very small starting genes

D) rapid separation and very small starting genes

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