NEET Mock Test Biotechnology

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HSA Natural Science HSST Botany MOCK TET

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🟥 TOPIC : BIO TECHNOLOGY

🟥 NUMBER OF QUESTIONS : 45

🟥 TIME : 20 MINS

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119

BIO TECHNOLOGY

🟥 TOPIC : BIO TECHNOLOGY

🟥 NUMBER OF QUESTIONS : 45

🟥 TIME : 20 MINS

1 / 45

1) Which one of the following bacteria has found extensive use in genetic engineering work in plants?

A) Clostridium septicum

B) Agrobacterium tumefaciens

C) Bacillus coagulens

D) Xanthomonas citri

2 / 45

2) Which vector can clone only a small fragment of DNA?

A) Bacterial artificial chromosome

B) Plasmid

C) Yeast artificial chromosome

D) Cosmid

3 / 45

3) Choose the correct pair from the following?

A) Polymerases - Break the DNA into fragments

B) Exonucleases - Make cuts at specific positions within DNA

C) Nucleases - Separate the two strands of DNA

D) Ligases - Join the two DNA molecules

4 / 45

4) Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called ?

A) bio-infringement

B) bioexploitation.

C) biodegradation

D) biopiracy

5 / 45

5) In gel electrophoresis, separated DNA fragments can be visualized with the help of ?

A) ethidium bromide in UV radiation

B) acetocarmine in UV radiation

C) ethidium bromide in infrared radiation.

D) acetocarmine in bright blue light

6 / 45

6) The Taq polymerase enzyme is obtained from ?

A) Thiobacillus ferroxidans.

B) Thermus aquaticus

C) Pseudomonas putida

D) Bacillus subtilis

7 / 45

7) A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using ?

A) Taq polymerase

B) EcoRI

C) ligase

D) polymerase III

8 / 45

8) Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

A) Genetic Engineering Approval Committee

B) Bio-safety committee

C) Indian Council of Agricultural Research

D) Research Committee on Genetic Manipulation

9 / 45

9) Which of the following is not a component of downstream processing?

A) Purification

B) Separation

C) Expression

D) Preservation

10 / 45

10) Which of the following is not a feature of the plasmids?

A) Circular structure

B) Single-stranded

C) Transferable

D) Independent replication

11 / 45

11) Golden rice is a transgenic crop of the future with the following improved trait?

A) high lysine (essential amino acid) content

B) high vitamin-A content.

C) insect resistance

D) high protein content

12 / 45

12) A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to ____?

A) Basmati

B) Sharbati Sonora

C) Co-667

D) Lerma Rojo

13 / 45

13) The process of RNA interference (RNAi) has been used in the development of plants resistant to __?

A) insects.

B) viruses

C) nematodes

D) fungi

14 / 45

14) Human insulin is being commercially produced from a transgenic species of __?

A) Mycobacterium

B) Saccharomyces

C) Rhizobium

D) Escherichia

15 / 45

15) Plasmid has been used as vector because __?

A) it can move between prokaryotic and eukaryotic cells

B) both ends show replication

C) it has antibiotic resistance gene.

D) it is circular DNA which have capacity to join to eukaryotic DNA

16 / 45

16) Biolistics (gene-gun) is suitable for ___?

A) DNA fingerprinting.

B) disarming pathogen vectors

C) constructing recombinant DNA by joining with vectors

D) transformation of plant cells

17 / 45

17) Which one of the following is a case of wrong matching ?

A) Somatic – Fusion of two hybridization diverse cells

B) Micropropagation – In vitro production of plants in large numbers

C) Vector DNA – Site for tRNA synthesis

D) Callus – Unorganized mass of cells produced in tissue culture

18 / 45

18) The linking of antibiotic resistance gene with the plasmid vector became possible with __?

A) DNA ligase

B) DNA polymerase

C) exonucleases

D) endonucleases

19 / 45

19) Which one of the following techniques made it possible to genetically engineer living organisms?

A) Recombinant DNA techniques

B) X-ray diffraction

C) Hybridization

D) Heavier isotope labelling

20 / 45

20) What is antisense technology ___?

A) RNA polymerase producing DNA

B) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

C) Production of somaclonal variants in tissue cultures

D) A cell displaying a foreign antigen used for synthesis of antigens

21 / 45

21) Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein___?

A) is activated by acid pH of the foregut of the insect pest

B) does not kill the carrier bacterium which is itself resistant to this toxin.

C) is coded by several genes including the gene cry

D) binds with epithelial cells of midgut of the insect pest ultimately killing it

22 / 45

22) Genetic engineering is possible, because ___?

A) we can see DNA by electron microscope

B) restriction endonucleases purified from bacteria can be used in vitro

C) we can cut DNA at specific sites by endonucleases like DNase I

D) the phenomenon of transduction in bacteria is well understood

23 / 45

23) Which one of the following is now being commercially produced by biotechnological procedures ___?

A) Quinine

B) Morphine

C) Nicotine

D) Insulin

24 / 45

24) The linking of antibiotic resistance gene with the plasmid vector became possible with (NCERT XII:197)

A) DNA ligase

B) DNA polymerase

C) exonucleases

D) endonucleases

25 / 45

25) Which one of the following techniques made it possible to genetically engineer living organisms? (NCERT XII :194)

A) X-ray diffraction

B) Recombinant DNA techniques

C) Hybridization

D) Heavier isotope labelling

26 / 45

26) What is antisense technology? (NCERT XII:209)

A) RNA polymerase producing DNA

B) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

C) Production of somaclonal variants in tissue cultures

D) A cell displaying a foreign antigen used for synthesis of antigens

27 / 45

27) Genetic engineering is possible, because (NCERT XII:194)

A) we can see DNA by electron microscope

B) restriction endonucleases purified from bacteria can be used in vitro

C) we can cut DNA at specific sites by endonucleases like DNase I

D) the phenomenon of transduction in bacteria is well understood

28 / 45

28) Which one of the following is now being commercially produced by biotechnological procedures? (NCERT XII:211)

A) Quinine

B) Morphine

C) Nicotine

D) Insulin

29 / 45

29) Maximum number of existing transgenic animals is of (NCERT XII:212)

A) mice

B) fish

C) cow

D) pig.

30 / 45

30) Gel electrophoresis is used for (NCERT XII:198)

A) cutting of DNA into fragments

B) isolation of DNA molecules

C) construction of recombinant DNA by joining with cloning vectors

D) separation of DNA fragments according to their size.

31 / 45

31) Restriction endonucleases are

A) used for in vitro DNA synthesis

B) used in genetic engineering

C) present in mammalian cells for degradation of DNA.

D) synthesized by bacteria

32 / 45

32) Restriction endonucleases are

A) used for in vitro DNA synthesis

B) used in genetic engineering

C) present in mammalian cells for degradation of DNA.

D) synthesized by bacteria

33 / 45

33) In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is (NCERT XII : 213)

A) Indian Council of Medical Research (ICMR)

B) Genetic Engineering Appraisal Committee (GEAC).

C) Research Committee on Genetic Manipulation (RCGM)

D) Council for Scientific and Industrial Research (CSIR)

34 / 45

34) The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of (NCERT XII:200)

A) insertional inactivation of alpha galactosidase in non-recombinant bacteria.

B) inactivation of glycosidase enzyme in recombinant bacteria

C) insertional inactivation of alpha galactosidase in recombinant bacteria

D) non-recombinant bacteria containing beta galactosidase

35 / 45

35) The process of separation and purification of expressed protein before marketing is called (NCERT XII:204)

A) upstream processing

B) bioprocessing

C) postproduction processing

D) downstream processing

36 / 45

36) Which one is a true statement regarding DNA polymerase used in PCR? (NCERT XII:203)

A) It is used to ligate introduced DNA in recipient cells.

B) It is isolated from a virus.

C) It remains active at high temperature.

D) It serves as a selectable marker.

37 / 45

37) The two polypeptides of human insulin are linked together by (NCERT XII:211)

A) phosphodiester bond.

B) hydrogen bonds

C) disulphide bridges

D) covalent bond

38 / 45

38) Two bacteria found to be very useful in genetic engineering experiments are

A) Nitrosomonas and Klebsiella

B) Escherichia and Agrobacterium.

C) Nitrobacter and Azotobacter

D) Rhizobium and Diplococcus

39 / 45

39) Stirred-tank bioreactors have been designed for (NCERT XII:204)

A) ensuring anaerobic conditions in the culture vessel

B) availability of oxygen throughout the process.

C) purification of the product

D) addition of preservatives to the product

40 / 45

40) Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (NCERT XII:200)

A) Ti plasmid

B) pBR322

C) λ phage

D) Retrovirus

41 / 45

41) A gene whose expression helps to identify transformed cell is known as (NCERT XII:199)

A) structural gene

B) plasmid

C) vector

D) selectable marker.

42 / 45

42) The first clinical gene therapy was given for treating (NCERT XII:211)

A) diabetes mellitus

B) adenosine deaminase deficiency.

C) rheumatoid arthritis

D) chicken pox

43 / 45

43) Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.(NCERT XII:196)

A) The enzyme cuts DNA molecule at identified position within the DNA.

B) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

C) The enzyme binds DNA at specific sites and cuts only one of the two strands.

D) The enzyme recognises a specific palindromic nucleotide sequence in the DNA.

44 / 45

44) The genetically-modified (GM) brinjal in India has been developed for (NCERT XII : 208)

A) enhancing mineral content

B) insect-resistance

C) drought-resistance.

D) enhancing shelf life

45 / 45

45) In RNAi, the genes are silenced using (NCERT XII:209)

A) ds-DNA.

B) ss-DNA

C) ss-RNA

D) ds-RNA

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